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Watch the Croutons– Keep a close eye on the croutons as they bake because cook times vary depending on the size of the bread cubes, how dry the loaf is, and oven temperatures

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The overnight culture was then diluted 1:100 in Mueller–Hinton broth and 200 µl applied to sterile Whatman cyclopore circular membrane 0.2 µm (Whatman plc, Maidstone, UK) placed on Mueller-Hinton agar plates (Sigma-Aldrich Company Ltd, Kent, UK) at 37 °C for 24 h to form colony biofilms

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